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13.37 > Academic > perlprimer (1.1.21)

PerlPrimer is a free, open-source GUI application written in Perl
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.

PerlPrimer's current features include the following:

- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry

For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472

This requires perl-tk and libwww-perl. For Real Time PCR, spidey is
required.

Maintained by: Petar Petrov
Approved by: rworkman
Keywords: perlprimer,pcr,primers,primer design,biology

Homepage:
http://perlprimer.sourceforge.net/

Source Downloads:
perlprimer-1.1.21.tar.bz2 (016eb870b988a280cdb730a86dd67591)

Download SlackBuild:
perlprimer.tar.gz
perlprimer.tar.gz.asc (FAQ)

(the SlackBuild does not include the source)

See our HOWTO for instructions on how to use the contents of this repository.

Access to the repository is available via:
ftp git cgit http rsync

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