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MACS: Model-based Analysis of ChIP-Seq

Next generation parallel sequencing technologies made chromatin
immunoprecipitation followed by sequencing (ChIP-Seq) a popular
strategy to study genome-wide protein-DNA interactions, while creating
challenges for analysis algorithms. We present Model-based Analysis of
ChIP-Seq (MACS) on short reads sequencers such as Genome Analyzer
(Illumina / Solexa). MACS empirically models the length of the
sequenced ChIP fragments, which tends to be shorter than sonication or
library construction size estimates, and uses it to improve the
spatial resolution of predicted binding sites. MACS also uses a
dynamic Poisson distribution to effectively capture local biases in
the genome sequence, allowing for more sensitive and robust
prediction. MACS compares favorably to existing ChIP-Seq peak-finding
algorithms, is publicly available open source, and can be used for
ChIP-Seq with or without control samples.

Maintained by: Petar Petrov
Keywords: biology,ChIP,cistrome
ChangeLog: cistrome-MACS


Source Downloads:
MACS-1.4.2-1.tar.gz (950dab09fe1335c8bbb34a896c21e3e2)

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cistrome-MACS.tar.gz.asc (FAQ)

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