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14.1 > Academic > perlprimer (1.1.21)

PerlPrimer is a free, open-source GUI application written in Perl 
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.

PerlPrimer's current features include the following:

- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry

An optional run-time dependency is spidey, for Real Time PCR.

For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472

This requires: perl-tk, libwww-perl

Maintained by: Petar Petrov
Keywords: perlprimer,pcr,primers,primer design,biology

Homepage:
http://perlprimer.sourceforge.net/

Source Downloads:
perlprimer-1.1.21.tar.bz2 (016eb870b988a280cdb730a86dd67591)

Download SlackBuild:
perlprimer.tar.gz
perlprimer.tar.gz.asc (FAQ)

(the SlackBuild does not include the source)

Validated for Slackware 14.1

See our HOWTO for instructions on how to use the contents of this repository.

Access to the repository is available via:
ftp git cgit http rsync

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